Bcdat medium

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WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence … WebOct 23, 2024 · A microdevice with protonema cells was submerged in BCDAT medium to prevent liquid evaporation from the channels; the dish was sealed with parafilm and left for 3–4 d at 25 °C under continuous ...

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WebJan 26, 2024 · After regeneration on PRMB medium containing 3 µM PABA, four-day-old transformed protoplasts were selected on BCDAT medium containing 3 µM PABA, 100 mg/l gentamicin, and 20 mg/l G418 for one week. Transformants were picked to BCDAT+PABA medium, then 10 days later a small clump of each was transferred to … WebJun 1, 2016 · The body plan of the early-diverged moss Physcomitrella patens (P. patens) is relatively simple. The vegetative gametophyte is composed of only a few cell types and developmental stages. Auxin was … is a manatee an animal

Metabolic Control of Gametophore Shoot Formation

WebJan 28, 2024 · GFP-mTalin transformants for cdka-dko deficiency were selected on BCDAT medium containing hygromycin (30 mg/liter) or zeocin (100 mg/liter) and further confirmed by polarotropism defects under polarized light. To observe cp-actin, a confocal laser scanning microscope was used (Zeiss LSM 510). The objective lens was a Plan … WebMar 30, 2024 · BCD medium (BCDAT without ammonium tartrate) may alternatively be used to grow and observe gametophore tissues. 3. This medium can be used several … WebSep 12, 2024 · They were then moved to BCDAT plates supplemented with 50 μg/ml hygromycin by transfer of the cellophane discs. After 10-14 days, cellophane disks were transferred to BCDAT medium without antibiotic. After an additional 10-14 days, growing colonies were transferred back to BCDAT plates supplemented with hygromycin for a … is a manatee a whale

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Growth-responses of protonemata of P. patens WT, ppnse4-4 …

WebDec 1, 2024 · All plant materials were cultured on BCDAT medium with 1 mM CaCl 2 at 25℃ under a 16 h/8 h (light/dark) photoperiod with the light intensity of 60−80 μmol … WebJul 3, 2024 · Selection plates are prepared by melting BCDAT medium, cooling to 55 °C, adding antibiotic (e.g., 15 μg/mL hygromycin), and pouring about 25 mL each into … olivia frazer teacherWebDec 7, 2015 · Transformants were confirmed after three rounds of selection on BCDAT medium supplemented with G418 medium (30 μg·mL −1) alternatively for 15 days each … is amana the same as kenmore

"WebJun 1, 2024 · Finally, half of the gametophores of S0, S1 and R1 were used for total RNA extraction, and half of R1 gametophores were transferred to BCDAT medium for continued growth. Arabidopsis thaliana ( A. thaliana ) seeds, ecotype Col-0 were washed with 75% Ethanol for 5 min, 5% sodium hypochlorite for 10 min, then washed with water for 10 … " - Bcdat medium

Bcdat medium

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WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence images of two or three leaves of ... WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark …

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WebNov 18, 2024 · BCDAT medium is composed of 1 mM MgSO 4, 1.84 mM KH 2 PO 4 (pH 6.5), 10 mM KNO 3, 45 μM FeSO 4, trace element solution (0.22 μM CuSO 4, 10 μM H 3 … WebAll strains were cultured either as 'spot inocula' on BCD agar medium supplemented with 1 mM CaCl 2 and 5 mM ammonium tartrate (BCDAT medium) or as lawns of protonema …

WebJun 1, 2016 · (E–H) Plants grown for one month on BCDAT medium to promote filamentous growth. (E, F) WT plant grown without auxin or with 12.5 μM NAA, respectively. (G) iaa2 … WebMay 20, 2016 · Chloronema-to-caulonema conversion takes place more rapidly on the BCD medium than on the BCDAT medium. 19. It is convenient to keep some BCD agar media in a 1.5 ml tube, since the tube can be heated in the heat block and the medium becomes molten quickly (100 °C, 2 min). 20. A thinner layer of the agar medium is better for clear …

WebDec 12, 2024 · Three-week-old protonemata of WT, ko lines and complemented lines grown on BCDAT medium were observed using a light microscope. Phenotypes of protonemal cells were observed in WT, ko lines and complemented lines. (a) ppkp4-1, ppkp4-2 and ppkp4-1/2 knockout mutants showed dead cells in protonemata. WebMar 29, 2015 · BCDAT medium is mostly similar to BCDAT, but growth of protonemata is a bit slower. We use BCDAT for selection with G418 and/or hygromycin after …

WebBCD medium. Do not include CaCl 2 if the medium is to be used to prepare protoplast regeneration media (PRMB, PRMT, or PRML). If using CaCl 2, add after autoclaving, …

WebOn minimal BCD medium, protonemal growth was strongly reduced and eventually ceased after a month (Figure 2A, C and G). At this stage, colonies comprised both chloronemal and caulonemal cells and... olivia free fontWebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. … is amana the same as goodmanWebSep 1, 2012 · A mixture of chloronemata and caulonemata was homogenized by vortexing for 1 minute using a six-well tube and ceramic balls (KURABO, Osaka, Japan), and vegetatively propagated on a BCDAT medium agar plate for four days. Then, the tissues were treated with or without phytohormones. olivia food shelfWebFeb 6, 2024 · Protonemata were dispersed in liquid BCDAT medium containing bleomycin or MMS and treated for 1 h. After treatment and washing recovered protonemata were inoculated as eight explants per quadrant of a Petri dish with drug-free BCDAT agar without cellophane overlay and left to grow. olivia freestanding bathWebSep 20, 2024 · The moss leaf cells were cultured in BCDAT medium. Liberalities, the information entropy of transcriptome were measured in each culture time. Figure 2: Bar charts of leaf cells transcriptome during reprogramming. The occupation rate of genes in a transcriptome was plotted in a bar chart. Heights of boxes in a bar chart indicate the … is a man bun professionalWebAfter bleomycin treatment, tissue was thoroughly rinsed in water, blotted on filter paper and either flash-frozen in liquid N 2 (repair t = 0) or left to recover on plates in liquid BCDAT medium ... olivia free fireWebDec 1, 2024 · Gametophore colonies grown on BCDAT medium for 40 days were used for the analysis of phenotypic characterization. The Branching patterns and the number of each module classes were measured according to the branching forms as previously described [23]. The lengths of internode were measured using ImageJ. 2.7. is a manatee a sea cow