WebDescription: Buforin II is a histone-derived antimicrobial peptide that readily translocates across lipid membranes without causing significant membrane permeabilization. Previous studies showed that mutating the sole proline of buforin II dramatically decreases its translocation. As well, researchers have proposed that the peptide crosses ... WebApr 26, 2024 · After the tumor volume reached by 50 mm 3, 50 nmol buforin IIb or injection buffer (DMSO in 100 ml PBS) as control were injected by tail vein injection every 2 days. The tumor volume and tumor weight were significantly inhibited in the buforin IIb peptide-treated mice compared with control from days 6 to 21 of treatment ( P < 0.05; Figure 4 (A ...
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WebJun 11, 2013 · Cell-penetrating peptides (CPPs) have proven very effective as intracellular delivery vehicles for various therapeutics. However, there are some concerns about non-specific penetration and cytotoxicity of CPPs for effective cancer treatments. Herein, based on the cell-penetrating motif of an anticancer peptide, buforin IIb, we designed several … WebJan 5, 1996 · The amino acid sequence of buforin I was identical in 37 of 39 amino-terminal residues of Xenopus histone H2A. The buforin I showed strong antimicrobial activitiesin vitroagainst a broad-spectrum of microorganisms and was found to be more potent than magainin 2. In addition, a 21-amino acid peptide, named buforin II, which was derived …
WebFurthermore, a recent E. coli expression system using green fluorescent protein (GFP) as a scaffold showed the efficient production of AMPs, such as protegrin-1 and PMAP-36 from pig, buforin-2 from toad and bactridin-1 from scorpion venom, in high yields . WebApr 27, 2013 · In the present study, the antimicrobial peptides BF2-A and BF2-C, two analogues of Buforin 2, were chemically synthesized and the activities were assayed. …
WebApr 4, 2024 · (A) Profile of the expressed buforin I in E. coli BL21 (DE3) cells at different sampling times; (B) Western blot analysis of the buforin I. M) protein ladder, L1) 6 h, L2) … WebFeb 28, 2024 · Here, we conjugated the antimicrobial CPP buforin II (BUF2) to the surface of magnetite nanoparticles to enhance their cell penetration. Conjugates were characterized via Fourier transform infrared spectroscopy, dynamic light scattering, and thermogravimetric analysis, and their biocompatibility was corroborated.
WebJul 11, 2000 · Buforin II is a 21-aa potent antimicrobial peptide that forms, in a hydrophobic medium, an amphipathic structure consisting of an N-terminal random coil region …
WebOct 15, 2012 · 2.1. Haemolymph Collection. Live C. madrasensis and M. casta were collected from Vembanad estuary, Kochi (Kerala, India) and S. cucullata, F. gracilis, and B. vittata from the coastal waters of Kochi. Samples were transported to laboratory in live condition. Haemolymph was collected from byssus muscles of bivalves and foot region of … black and mild shorts cigarsWebOct 5, 2024 · stability in the presence of physiological salts compared to buforin 2. The MIC va lues of A. baumannii KCTC 250 8 and A. baumannii 907233 strain were 4 and 2 µM, respectively. black and mild sweetsWebApr 27, 2013 · In the present study, the antimicrobial peptides BF2-A and BF2-C, two analogues of Buforin 2, were chemically synthesized and the activities were assayed. To elucidate the bactericidal mechanism of BF2-A/C and their different antimicrobial activities, the influence of peptides to E. coli cell membrane and targets of intracellular action were … black and mild sweets wood tipWebJun 18, 2024 · The solution structure of buforin-2 is composed largely of an α-helix spanning from Gly 7 to Arg 20 (amino acids 22–35 of buforin-1) with a small distortion caused by the Pro 11 hinge (amino acid 26 of buforin-1). Thus, the Q-F bond between amino acids 24 and 25 of buforin-1 may reside in the hinge region of the helix. This α … black and mild seafood feastWebAug 19, 2012 · 2. Materials and Methods 2.1. Haemolymph Collection. Live C. madrasensis and M. casta were collected from Vembanad estuary, Kochi (Kerala, ... 25 amino acid sequence of the deduced peptide showed similarity to previously reported histone-H2A-derived AMPs like Buforin I, Buforin II, Hipposin, Himanturin, Abhisin, ... black and milds wineWebOct 5, 2012 · Unlike magainin 2 amide, a prototypical membrane disruptive AMP, buforin II amide adopts a poorly helical structure in membranes closely mimicking the composition of Gram negative bacteria, such as Escherichia coli, and binds to a short duplex DNA sequence with high affinity, ultimately forming peptide–DNA condensates. ... black and mild sweets flavorWebDec 24, 2024 · The emergence of drug-resistant bacteria emphasizes the urgent need for novel antibiotics. The antimicrobial peptide TS shows extensive antibacterial activity in vitro and in vivo, especially in gram-negative bacteria; however, its antibacterial mechanism is unclear. Here, we find that TS without hemolytic activity disrupts the integrity of the outer … black and mild tobacco